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electrophysiology pipette puller p-1000  (Sutter Instrument Company)
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( A ) Schematic detailing pAAV-ChR2-EYFP injection unilaterally into POm ( Right ), and optogenetic stimulation of POm-striatal afferents whilst recording from identified and unidentified neurons via ex vivo slice of posterior DLS (AP range: –0.34 to –1.22 relative to Bregma; Left ). See . Illumination (2.5ms pulses of 470 nm light,~0.6 mW intensity) was delivered through the 40 x objective. ( B ) Representative injection site (orange) in POm ( Left ), and viral spread of all <t>electrophysiology</t> injections within highlighted POm (purple; Right ). S1BF = S1 Barrel Field. Scale = 1 mm. ( C ) Red box inset from panel ( B ) highlighting stereotypical POm-cortical projection pattern to S1BF L1 and L5a ( ; ; ). Right : POm-striatal axons within posterior DLS. CC = corpus callosum. Scale = 200 µm. ( D ) Representative cell type-specific PSPs to SP stimulation. Colored lines = average PSP of 20 sweeps. Gray lines = 20 individual traces. Solid vertical and dashed horizontal lines = latency and amplitude, respectively. Red dashed line = 0 mV. Blue tick = photostimulation (PS). Time scale = 10ms. Voltage scale = 4 mV. ( E ) Amplitudes evoked by each cell type were similar (D1-SPNs=20 cells from 6 mice, D2-SPNs=11 cells from 5 mice, PVs = 17 cells from 7 mice, unidentified SPNs = 7 cells from 4 mice). Inset shows grand average PSPs. Time scale = 10ms. Voltage scale = 2 mV. ( F ) Latency to maximum PSP amplitude is significantly quicker in PVs than all other cell types. ( G–H ) Representative responses of ( G ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( H ) PV ( Top ) and putative SPN ( Bottom ) to PPR stimulation . PPR is defined as the ratio of PSP amplitude of pulse 2 over the ratio of PSP amplitude of pulse 1. PPR PS parameters = five 2.5ms pulses with 50ms interpulse intervals (20 Hz). Time scale = 100ms. Voltage scale = 2 mV. See Stimulation of POm-striatal afferents evokes similar PPR responses. ( J–K ) Representative responses of ( J ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( K ) PV ( Top ) and putative SPN ( Bottom ) to train stimulation . Colored lines = average of five individual gray traces. Train PS parameters = thirty 2.5ms pulses with 64.2ms interpulse intervals (15 Hz). Time scale = 1000ms. Voltage scale = 2 mV. ( L ) Relative PSP amplitude (average of pulses 5–15 compared to pulse 1) is significantly larger than both SPNs. Data are mean ± SEM. *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
Electrophysiology Pipette Puller P 1000, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal pipette puller p- 1000  (Sutter Instrument Company)
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( A ) Schematic detailing pAAV-ChR2-EYFP injection unilaterally into POm ( Right ), and optogenetic stimulation of POm-striatal afferents whilst recording from identified and unidentified neurons via ex vivo slice of posterior DLS (AP range: –0.34 to –1.22 relative to Bregma; Left ). See . Illumination (2.5ms pulses of 470 nm light,~0.6 mW intensity) was delivered through the 40 x objective. ( B ) Representative injection site (orange) in POm ( Left ), and viral spread of all <t>electrophysiology</t> injections within highlighted POm (purple; Right ). S1BF = S1 Barrel Field. Scale = 1 mm. ( C ) Red box inset from panel ( B ) highlighting stereotypical POm-cortical projection pattern to S1BF L1 and L5a ( ; ; ). Right : POm-striatal axons within posterior DLS. CC = corpus callosum. Scale = 200 µm. ( D ) Representative cell type-specific PSPs to SP stimulation. Colored lines = average PSP of 20 sweeps. Gray lines = 20 individual traces. Solid vertical and dashed horizontal lines = latency and amplitude, respectively. Red dashed line = 0 mV. Blue tick = photostimulation (PS). Time scale = 10ms. Voltage scale = 4 mV. ( E ) Amplitudes evoked by each cell type were similar (D1-SPNs=20 cells from 6 mice, D2-SPNs=11 cells from 5 mice, PVs = 17 cells from 7 mice, unidentified SPNs = 7 cells from 4 mice). Inset shows grand average PSPs. Time scale = 10ms. Voltage scale = 2 mV. ( F ) Latency to maximum PSP amplitude is significantly quicker in PVs than all other cell types. ( G–H ) Representative responses of ( G ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( H ) PV ( Top ) and putative SPN ( Bottom ) to PPR stimulation . PPR is defined as the ratio of PSP amplitude of pulse 2 over the ratio of PSP amplitude of pulse 1. PPR PS parameters = five 2.5ms pulses with 50ms interpulse intervals (20 Hz). Time scale = 100ms. Voltage scale = 2 mV. See Stimulation of POm-striatal afferents evokes similar PPR responses. ( J–K ) Representative responses of ( J ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( K ) PV ( Top ) and putative SPN ( Bottom ) to train stimulation . Colored lines = average of five individual gray traces. Train PS parameters = thirty 2.5ms pulses with 64.2ms interpulse intervals (15 Hz). Time scale = 1000ms. Voltage scale = 2 mV. ( L ) Relative PSP amplitude (average of pulses 5–15 compared to pulse 1) is significantly larger than both SPNs. Data are mean ± SEM. *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
Horizontal Pipette Puller P 1000, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal pipette puller p-1000  (Sutter Instrument Company)
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Sutter Instrument Company horizontal pipette puller p-1000
( A ) Schematic detailing pAAV-ChR2-EYFP injection unilaterally into POm ( Right ), and optogenetic stimulation of POm-striatal afferents whilst recording from identified and unidentified neurons via ex vivo slice of posterior DLS (AP range: –0.34 to –1.22 relative to Bregma; Left ). See . Illumination (2.5ms pulses of 470 nm light,~0.6 mW intensity) was delivered through the 40 x objective. ( B ) Representative injection site (orange) in POm ( Left ), and viral spread of all <t>electrophysiology</t> injections within highlighted POm (purple; Right ). S1BF = S1 Barrel Field. Scale = 1 mm. ( C ) Red box inset from panel ( B ) highlighting stereotypical POm-cortical projection pattern to S1BF L1 and L5a ( ; ; ). Right : POm-striatal axons within posterior DLS. CC = corpus callosum. Scale = 200 µm. ( D ) Representative cell type-specific PSPs to SP stimulation. Colored lines = average PSP of 20 sweeps. Gray lines = 20 individual traces. Solid vertical and dashed horizontal lines = latency and amplitude, respectively. Red dashed line = 0 mV. Blue tick = photostimulation (PS). Time scale = 10ms. Voltage scale = 4 mV. ( E ) Amplitudes evoked by each cell type were similar (D1-SPNs=20 cells from 6 mice, D2-SPNs=11 cells from 5 mice, PVs = 17 cells from 7 mice, unidentified SPNs = 7 cells from 4 mice). Inset shows grand average PSPs. Time scale = 10ms. Voltage scale = 2 mV. ( F ) Latency to maximum PSP amplitude is significantly quicker in PVs than all other cell types. ( G–H ) Representative responses of ( G ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( H ) PV ( Top ) and putative SPN ( Bottom ) to PPR stimulation . PPR is defined as the ratio of PSP amplitude of pulse 2 over the ratio of PSP amplitude of pulse 1. PPR PS parameters = five 2.5ms pulses with 50ms interpulse intervals (20 Hz). Time scale = 100ms. Voltage scale = 2 mV. See Stimulation of POm-striatal afferents evokes similar PPR responses. ( J–K ) Representative responses of ( J ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( K ) PV ( Top ) and putative SPN ( Bottom ) to train stimulation . Colored lines = average of five individual gray traces. Train PS parameters = thirty 2.5ms pulses with 64.2ms interpulse intervals (15 Hz). Time scale = 1000ms. Voltage scale = 2 mV. ( L ) Relative PSP amplitude (average of pulses 5–15 compared to pulse 1) is significantly larger than both SPNs. Data are mean ± SEM. *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
Horizontal Pipette Puller P 1000, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pipette puller p-1000 sutter instrument  (Sutter Instrument Company)
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( A ) Schematic detailing pAAV-ChR2-EYFP injection unilaterally into POm ( Right ), and optogenetic stimulation of POm-striatal afferents whilst recording from identified and unidentified neurons via ex vivo slice of posterior DLS (AP range: –0.34 to –1.22 relative to Bregma; Left ). See . Illumination (2.5ms pulses of 470 nm light,~0.6 mW intensity) was delivered through the 40 x objective. ( B ) Representative injection site (orange) in POm ( Left ), and viral spread of all <t>electrophysiology</t> injections within highlighted POm (purple; Right ). S1BF = S1 Barrel Field. Scale = 1 mm. ( C ) Red box inset from panel ( B ) highlighting stereotypical POm-cortical projection pattern to S1BF L1 and L5a ( ; ; ). Right : POm-striatal axons within posterior DLS. CC = corpus callosum. Scale = 200 µm. ( D ) Representative cell type-specific PSPs to SP stimulation. Colored lines = average PSP of 20 sweeps. Gray lines = 20 individual traces. Solid vertical and dashed horizontal lines = latency and amplitude, respectively. Red dashed line = 0 mV. Blue tick = photostimulation (PS). Time scale = 10ms. Voltage scale = 4 mV. ( E ) Amplitudes evoked by each cell type were similar (D1-SPNs=20 cells from 6 mice, D2-SPNs=11 cells from 5 mice, PVs = 17 cells from 7 mice, unidentified SPNs = 7 cells from 4 mice). Inset shows grand average PSPs. Time scale = 10ms. Voltage scale = 2 mV. ( F ) Latency to maximum PSP amplitude is significantly quicker in PVs than all other cell types. ( G–H ) Representative responses of ( G ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( H ) PV ( Top ) and putative SPN ( Bottom ) to PPR stimulation . PPR is defined as the ratio of PSP amplitude of pulse 2 over the ratio of PSP amplitude of pulse 1. PPR PS parameters = five 2.5ms pulses with 50ms interpulse intervals (20 Hz). Time scale = 100ms. Voltage scale = 2 mV. See Stimulation of POm-striatal afferents evokes similar PPR responses. ( J–K ) Representative responses of ( J ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( K ) PV ( Top ) and putative SPN ( Bottom ) to train stimulation . Colored lines = average of five individual gray traces. Train PS parameters = thirty 2.5ms pulses with 64.2ms interpulse intervals (15 Hz). Time scale = 1000ms. Voltage scale = 2 mV. ( L ) Relative PSP amplitude (average of pulses 5–15 compared to pulse 1) is significantly larger than both SPNs. Data are mean ± SEM. *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
Pipette Puller P 1000 Sutter Instrument, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Schematic detailing pAAV-ChR2-EYFP injection unilaterally into POm ( Right ), and optogenetic stimulation of POm-striatal afferents whilst recording from identified and unidentified neurons via ex vivo slice of posterior DLS (AP range: –0.34 to –1.22 relative to Bregma; Left ). See . Illumination (2.5ms pulses of 470 nm light,~0.6 mW intensity) was delivered through the 40 x objective. ( B ) Representative injection site (orange) in POm ( Left ), and viral spread of all <t>electrophysiology</t> injections within highlighted POm (purple; Right ). S1BF = S1 Barrel Field. Scale = 1 mm. ( C ) Red box inset from panel ( B ) highlighting stereotypical POm-cortical projection pattern to S1BF L1 and L5a ( ; ; ). Right : POm-striatal axons within posterior DLS. CC = corpus callosum. Scale = 200 µm. ( D ) Representative cell type-specific PSPs to SP stimulation. Colored lines = average PSP of 20 sweeps. Gray lines = 20 individual traces. Solid vertical and dashed horizontal lines = latency and amplitude, respectively. Red dashed line = 0 mV. Blue tick = photostimulation (PS). Time scale = 10ms. Voltage scale = 4 mV. ( E ) Amplitudes evoked by each cell type were similar (D1-SPNs=20 cells from 6 mice, D2-SPNs=11 cells from 5 mice, PVs = 17 cells from 7 mice, unidentified SPNs = 7 cells from 4 mice). Inset shows grand average PSPs. Time scale = 10ms. Voltage scale = 2 mV. ( F ) Latency to maximum PSP amplitude is significantly quicker in PVs than all other cell types. ( G–H ) Representative responses of ( G ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( H ) PV ( Top ) and putative SPN ( Bottom ) to PPR stimulation . PPR is defined as the ratio of PSP amplitude of pulse 2 over the ratio of PSP amplitude of pulse 1. PPR PS parameters = five 2.5ms pulses with 50ms interpulse intervals (20 Hz). Time scale = 100ms. Voltage scale = 2 mV. See Stimulation of POm-striatal afferents evokes similar PPR responses. ( J–K ) Representative responses of ( J ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( K ) PV ( Top ) and putative SPN ( Bottom ) to train stimulation . Colored lines = average of five individual gray traces. Train PS parameters = thirty 2.5ms pulses with 64.2ms interpulse intervals (15 Hz). Time scale = 1000ms. Voltage scale = 2 mV. ( L ) Relative PSP amplitude (average of pulses 5–15 compared to pulse 1) is significantly larger than both SPNs. Data are mean ± SEM. *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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Image Search Results


( A ) Schematic detailing pAAV-ChR2-EYFP injection unilaterally into POm ( Right ), and optogenetic stimulation of POm-striatal afferents whilst recording from identified and unidentified neurons via ex vivo slice of posterior DLS (AP range: –0.34 to –1.22 relative to Bregma; Left ). See . Illumination (2.5ms pulses of 470 nm light,~0.6 mW intensity) was delivered through the 40 x objective. ( B ) Representative injection site (orange) in POm ( Left ), and viral spread of all electrophysiology injections within highlighted POm (purple; Right ). S1BF = S1 Barrel Field. Scale = 1 mm. ( C ) Red box inset from panel ( B ) highlighting stereotypical POm-cortical projection pattern to S1BF L1 and L5a ( ; ; ). Right : POm-striatal axons within posterior DLS. CC = corpus callosum. Scale = 200 µm. ( D ) Representative cell type-specific PSPs to SP stimulation. Colored lines = average PSP of 20 sweeps. Gray lines = 20 individual traces. Solid vertical and dashed horizontal lines = latency and amplitude, respectively. Red dashed line = 0 mV. Blue tick = photostimulation (PS). Time scale = 10ms. Voltage scale = 4 mV. ( E ) Amplitudes evoked by each cell type were similar (D1-SPNs=20 cells from 6 mice, D2-SPNs=11 cells from 5 mice, PVs = 17 cells from 7 mice, unidentified SPNs = 7 cells from 4 mice). Inset shows grand average PSPs. Time scale = 10ms. Voltage scale = 2 mV. ( F ) Latency to maximum PSP amplitude is significantly quicker in PVs than all other cell types. ( G–H ) Representative responses of ( G ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( H ) PV ( Top ) and putative SPN ( Bottom ) to PPR stimulation . PPR is defined as the ratio of PSP amplitude of pulse 2 over the ratio of PSP amplitude of pulse 1. PPR PS parameters = five 2.5ms pulses with 50ms interpulse intervals (20 Hz). Time scale = 100ms. Voltage scale = 2 mV. See Stimulation of POm-striatal afferents evokes similar PPR responses. ( J–K ) Representative responses of ( J ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( K ) PV ( Top ) and putative SPN ( Bottom ) to train stimulation . Colored lines = average of five individual gray traces. Train PS parameters = thirty 2.5ms pulses with 64.2ms interpulse intervals (15 Hz). Time scale = 1000ms. Voltage scale = 2 mV. ( L ) Relative PSP amplitude (average of pulses 5–15 compared to pulse 1) is significantly larger than both SPNs. Data are mean ± SEM. *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

Journal: eLife

Article Title: Role of posterior medial thalamus in the modulation of striatal circuitry and choice behavior

doi: 10.7554/eLife.98563

Figure Lengend Snippet: ( A ) Schematic detailing pAAV-ChR2-EYFP injection unilaterally into POm ( Right ), and optogenetic stimulation of POm-striatal afferents whilst recording from identified and unidentified neurons via ex vivo slice of posterior DLS (AP range: –0.34 to –1.22 relative to Bregma; Left ). See . Illumination (2.5ms pulses of 470 nm light,~0.6 mW intensity) was delivered through the 40 x objective. ( B ) Representative injection site (orange) in POm ( Left ), and viral spread of all electrophysiology injections within highlighted POm (purple; Right ). S1BF = S1 Barrel Field. Scale = 1 mm. ( C ) Red box inset from panel ( B ) highlighting stereotypical POm-cortical projection pattern to S1BF L1 and L5a ( ; ; ). Right : POm-striatal axons within posterior DLS. CC = corpus callosum. Scale = 200 µm. ( D ) Representative cell type-specific PSPs to SP stimulation. Colored lines = average PSP of 20 sweeps. Gray lines = 20 individual traces. Solid vertical and dashed horizontal lines = latency and amplitude, respectively. Red dashed line = 0 mV. Blue tick = photostimulation (PS). Time scale = 10ms. Voltage scale = 4 mV. ( E ) Amplitudes evoked by each cell type were similar (D1-SPNs=20 cells from 6 mice, D2-SPNs=11 cells from 5 mice, PVs = 17 cells from 7 mice, unidentified SPNs = 7 cells from 4 mice). Inset shows grand average PSPs. Time scale = 10ms. Voltage scale = 2 mV. ( F ) Latency to maximum PSP amplitude is significantly quicker in PVs than all other cell types. ( G–H ) Representative responses of ( G ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( H ) PV ( Top ) and putative SPN ( Bottom ) to PPR stimulation . PPR is defined as the ratio of PSP amplitude of pulse 2 over the ratio of PSP amplitude of pulse 1. PPR PS parameters = five 2.5ms pulses with 50ms interpulse intervals (20 Hz). Time scale = 100ms. Voltage scale = 2 mV. See Stimulation of POm-striatal afferents evokes similar PPR responses. ( J–K ) Representative responses of ( J ) D1-SPN ( Top ) and D2-SPN ( Bottom ), and ( K ) PV ( Top ) and putative SPN ( Bottom ) to train stimulation . Colored lines = average of five individual gray traces. Train PS parameters = thirty 2.5ms pulses with 64.2ms interpulse intervals (15 Hz). Time scale = 1000ms. Voltage scale = 2 mV. ( L ) Relative PSP amplitude (average of pulses 5–15 compared to pulse 1) is significantly larger than both SPNs. Data are mean ± SEM. *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

Article Snippet: Other , Electrophysiology Pipette Puller , Sutter Instruments , P-1000 , Equipment.

Techniques: Injection, Ex Vivo